Análisis histológico luego de la amputación de las puntas de los brazos en <italic>Ophioplocus januarii</italic> (Echinodermata: Ophiuroidea)

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doi:10.15517/rbt.v63i2.23164

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Artículo • Rev. biol. trop 63 (Suppl 2) • Jun 2015 • https://doi.org/10.15517/rbt.v63i2.23164 linkcopiar

Análisis histológico luego de la amputación de las puntas de los brazos en Ophioplocus januarii (Echinodermata: Ophiuroidea)

Autoría SCIMAGO INSTITUTIONS RANKINGS

Resumen

Se examinaron los cambios histológicos y morfológicos producidos en la punta del brazo de la ofiura Ophioplocus januarii luego de su amputación. En el presente trabajo se describe el proceso de cicatrización de la herida y se lo compara con otras especies de la misma Clase. Este proceso en O. januarii involucra el cierre de la herida, la reparación del tejido dañado y la posterior formación de un presunto blastema formado por células indiferenciadas, esclerocitos y presuntas células blastémicas. Los esclerocitos son las células más abundantes debido a que estas células son cruciales para la regeneración del esqueleto. Los resultados encontrados en este estudio apoyan los resultados que se encuentran en otras estrellas frágiles.

Palabras clave: estrella frágil; equinodermo; análisis histológico; regeneración; esclerocitos

Autoría

Gisele Di Giorgio

Laboratorio de Parasitología, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; digiorgio@cenpat.edu.arCONICETArgentinaPuerto Madryn, Chubut, ArgentinaLaboratorio de Parasitología, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; digiorgio@cenpat.edu.ar

Tamara Rubilar

LAPEMAR, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; rubilar@cenpat.edu.arCONICETArgentinaPuerto Madryn, Chubut, ArgentinaLAPEMAR, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; rubilar@cenpat.edu.ar

Martín I. Brogger

Laboratorio de Ecosistemas Costeros, MACN-CONICET, Av. Ángel Gallardo 470 (C1405DJR), Buenos Aires, Argentina; LARBIM, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; mbrogger@bg.fcen.uba.arCONICETArgentinaBuenos Aires, ArgentinaLaboratorio de Ecosistemas Costeros, MACN-CONICET, Av. Ángel Gallardo 470 (C1405DJR), Buenos Aires, Argentina; LARBIM, CENPAT-CONICET, Blvd. Brown 2915 (U9120ACD), Puerto Madryn, Chubut, Argentina; mbrogger@bg.fcen.uba.ar

SCIMAGO INSTITUTIONS RANKINGS

Figuras

Figuras (5)

Thumbnail

Fig. 1
Scanning Electronic Microscope images. Frontal view of the amputated arm tip at different times post amputation (dpa). (A) 2 dpa, denote the strongly concave shape (arrow) covered by the external skeletal shields and a thin epithelium. (B) 4 dpa, denote the flattened shape and the disorganized tissue (asterisk). (C) 6 dpa and (D) 8 dpa, denote the loss of the concave shape and the organized tissue (asterisk) and the calcium deposits (arrowhead). (E) 11 dpa and (F) 16 dpa, denote the ossicles in formation (arrows) and the growth of the arm (asterisk). AAP aboral arm plate, LS lateral shield, OAP oral arm plate, P podia, S spine. Scale bars: 500 µm.

Thumbnail

Fig. 2
Detail of cells involved in the regeneration process. (A) Coelomocytes (arrow). (B) Sclerocytes. (C) Granule cells. (D) Presumptive blastemic cells. Note the two nuclei of the cell (arrow). (E) Phagocytes. (F) Cells in mitosis. (G) Cells with cytoplasmic expansions. (H) Amoebocytes. (I) Cell in differentiation. Scale bars: 1: 5 μm; 2: 10 μm; 3: 20 μm.

Thumbnail

Fig. 3
Sagittal sections of the regenerating arm tip of Ophioplocus januarii. A-C 2 dpa. (A) Overview of the arm tip in regeneration. Denote the concave shape of the wound surface (arrowhead). (B) Detail of a. Denote the thin epithelium covering the wound. The central area is disorganized and discontinuous (arrowhead). The peripheral regions are more organized (arrow). (C) Detail of b. denotes the presence of various cell types; cells with cytoplasmic expansions (arrowhead) and phagocytes (arrow). D-F 4 dpa. (D) Overview of the arm tip in regeneration. Denote the smooth concave shape of the wound surface (arrowhead). (E) Detail of d. Denote the scar epithelium, is more organized and thicker (arrowhead). Under the epithelium there is a large amount of secreted fibers (arrow). (F) Detail of e. Denote the distal end of the channels sealed by sclerocytes. G-I 6 dpa. (G) Overview of the arm tip in regeneration. Denote the wound surface (arrowhead). (H) Detail of g, there are areas with high cellular density (arrowhead). (I) Detail of h. Denote the shape of the epithelium (arrow) and the more abundant cells (sclerocytes) (arrowhead). AAP aboral arms plate, C coelomic cavity, CT connective tissue, E epineural sinus, H hyponeural sinus, L interosciculars ligaments, M muscle, OAP oral arm plate, P podia, V vertebral ossicle. Scale bars: 1: 200 μm, 2: 50 μm, 3: 10 μm, 4: 500 μm; 5: 100 μm.

Thumbnail

Fig. 4
Sagittal section of the regenerating arm tip of Ophioplocus januarii. A-C 8 dpa. (A) Overview of the arm tip in regeneration. Denote the straight shape of the wound surface (arrowhead). (B) Detail of c. Denote the injured surface with intense cellular activity, immersed in a dense disorganized connective tissue (arrow). (C) Detail of b. Denote the large cell accumulation in close association with the nerve. D-F 16 dpa. (D) Overview of the arm tip in regeneration. Denote the flattened shape of the wound surface (arrowhead). (E) Detail of d. Denote the formation of new sclerocytes at the aboral extremity of the wound (arrow). (F) Detail of e. Denote the organized epithelium (arrowhead) and the loose connective tissue fibers below it (arrow). G-I 20 dpa. (G) Overview of the arm tip in regeneration. Denote a protrusion in the oral surface (arrowhead). (H) Detail of g. Denote an accumulation of cells proximal to the nerve. (I) Detail of h. Denote the organized area formed by sclerocytes (arrowhead). AAP aboral arm plate, C coelomic cavity, CT connective tissue, E epineural sinus, G ganglion, H hyponeural sinus, L interosciculars ligaments, M muscle, N nerve, OAP oral arm plate, P podia, V vertebral ossicle. Scale bars: 1: 200 μm; 2: 50 μm; 3: 100 μm; 4: 20 μm.

Thumbnail

Fig. 5
Sagittal sections of the regenerating arm tip of Ophioplocus januarii. A-D 30 dpa. (A) Overview of the arm tip in regeneration. Denote the stalked presumptive blastema at the end of the arm, characterized by the presence of undifferentiated cells. (B) Detail of the stalked presumptive blastema. Denote the formation of trabeculae in the blastema area (arrowhead). (C) Detail of a, denote the abundance of presumptive blastemal cells (arrow) but also there are fibers of the connective tissue (arrowhead). (D) Detail of the stalked presumptive blastema. Denote presumptive blastemic cells (arrow) and sclerocytes (arrowhead). Scale bars: 1: 200 μm; 2: 50 μm; 3: 10 μm.

imageFig. 1 Scanning Electronic Microscope images. Frontal view of the amputated arm tip at different times post amputation (dpa). (A) 2 dpa, denote the strongly concave shape (arrow) covered by the external skeletal shields and a thin epithelium. (B) 4 dpa, denote the flattened shape and the disorganized tissue (asterisk). (C) 6 dpa and (D) 8 dpa, denote the loss of the concave shape and the organized tissue (asterisk) and the calcium deposits (arrowhead). (E) 11 dpa and (F) 16 dpa, denote the ossicles in formation (arrows) and the growth of the arm (asterisk). AAP aboral arm plate, LS lateral shield, OAP oral arm plate, P podia, S spine. Scale bars: 500 µm. open_in_new

imageFig. 2 Detail of cells involved in the regeneration process. (A) Coelomocytes (arrow). (B) Sclerocytes. (C) Granule cells. (D) Presumptive blastemic cells. Note the two nuclei of the cell (arrow). (E) Phagocytes. (F) Cells in mitosis. (G) Cells with cytoplasmic expansions. (H) Amoebocytes. (I) Cell in differentiation. Scale bars: 1: 5 μm; 2: 10 μm; 3: 20 μm. open_in_new

imageFig. 3 Sagittal sections of the regenerating arm tip of Ophioplocus januarii. A-C 2 dpa. (A) Overview of the arm tip in regeneration. Denote the concave shape of the wound surface (arrowhead). (B) Detail of a. Denote the thin epithelium covering the wound. The central area is disorganized and discontinuous (arrowhead). The peripheral regions are more organized (arrow). (C) Detail of b. denotes the presence of various cell types; cells with cytoplasmic expansions (arrowhead) and phagocytes (arrow). D-F 4 dpa. (D) Overview of the arm tip in regeneration. Denote the smooth concave shape of the wound surface (arrowhead). (E) Detail of d. Denote the scar epithelium, is more organized and thicker (arrowhead). Under the epithelium there is a large amount of secreted fibers (arrow). (F) Detail of e. Denote the distal end of the channels sealed by sclerocytes. G-I 6 dpa. (G) Overview of the arm tip in regeneration. Denote the wound surface (arrowhead). (H) Detail of g, there are areas with high cellular density (arrowhead). (I) Detail of h. Denote the shape of the epithelium (arrow) and the more abundant cells (sclerocytes) (arrowhead). AAP aboral arms plate, C coelomic cavity, CT connective tissue, E epineural sinus, H hyponeural sinus, L interosciculars ligaments, M muscle, OAP oral arm plate, P podia, V vertebral ossicle. Scale bars: 1: 200 μm, 2: 50 μm, 3: 10 μm, 4: 500 μm; 5: 100 μm. open_in_new

imageFig. 4 Sagittal section of the regenerating arm tip of Ophioplocus januarii. A-C 8 dpa. (A) Overview of the arm tip in regeneration. Denote the straight shape of the wound surface (arrowhead). (B) Detail of c. Denote the injured surface with intense cellular activity, immersed in a dense disorganized connective tissue (arrow). (C) Detail of b. Denote the large cell accumulation in close association with the nerve. D-F 16 dpa. (D) Overview of the arm tip in regeneration. Denote the flattened shape of the wound surface (arrowhead). (E) Detail of d. Denote the formation of new sclerocytes at the aboral extremity of the wound (arrow). (F) Detail of e. Denote the organized epithelium (arrowhead) and the loose connective tissue fibers below it (arrow). G-I 20 dpa. (G) Overview of the arm tip in regeneration. Denote a protrusion in the oral surface (arrowhead). (H) Detail of g. Denote an accumulation of cells proximal to the nerve. (I) Detail of h. Denote the organized area formed by sclerocytes (arrowhead). AAP aboral arm plate, C coelomic cavity, CT connective tissue, E epineural sinus, G ganglion, H hyponeural sinus, L interosciculars ligaments, M muscle, N nerve, OAP oral arm plate, P podia, V vertebral ossicle. Scale bars: 1: 200 μm; 2: 50 μm; 3: 100 μm; 4: 20 μm. open_in_new

imageFig. 5 Sagittal sections of the regenerating arm tip of Ophioplocus januarii. A-D 30 dpa. (A) Overview of the arm tip in regeneration. Denote the stalked presumptive blastema at the end of the arm, characterized by the presence of undifferentiated cells. (B) Detail of the stalked presumptive blastema. Denote the formation of trabeculae in the blastema area (arrowhead). (C) Detail of a, denote the abundance of presumptive blastemal cells (arrow) but also there are fibers of the connective tissue (arrowhead). (D) Detail of the stalked presumptive blastema. Denote presumptive blastemic cells (arrow) and sclerocytes (arrowhead). Scale bars: 1: 200 μm; 2: 50 μm; 3: 10 μm. open_in_new

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Universidad de Costa Rica Universidad de Costa Rica. Escuela de Biología, 2060 San José, Costa Rica, San Pedro, San José, CR, 2060, 2511-5500 , 2511-5550 - E-mail: rbt@biologia.ucr.ac.cr

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