Authors Year Study design Population Intervention Comparison Outcome Intervertebral disc Belavy et al. 2019 Observational, cross-sectional High volume road cyclist aged 25 to 35 (n= 36, 18 cyclist, 18 no sport) No intervention -No sport -Higher IVD's in cyclist (0.75 mm, p=0.006) -Higher IVD T2 times in cyclist (10.5 ms,p=0.021) Belavy et al. 2017 Observational, cross-sectional Individuals aged 25 to 35 years of age (n= 79, 24 no sport, 30 joggers,25 long distance runners) No intervention -No sport -Joggers (20-40 km) -Long distance runners (50+km) -Higher IVD T2 times in joggers (+9.2%) and long distance runners (+11.4%) (p<0.01 compared to no sport) -Higher IVD's relative to vertebral body in joggers and long distance runners (p<0.05 compared to no sport) Bowden et al. 2018 Observational, case control Participants aged 35 to 55 (n=26,15 daily vigorous activity, 16 high sedentarism, 14 more than 30 min of moderate to vigorous activity) No intervention -Daily vigoruous activity -High sedentary -More than 30 minutes of moderate to vigorous activity -Higher T2 values in T5/S1 (p=.004) in particpants with any amount of daily vigorous activity Khanzadeh et al. 2020 Quasi experimental Men with unilateral or bilateral lumbar and radicular leg pains due to lumbar disc herniation (n= 30, 15 suspension group, 15 conventional group) Suspension exercise (8 weeks) Conventional core stability exercise No change in IVD height between groups Owen et al. 2020 Single blinded RCT Individuals with non specific chronic low back pain (n=40, 20 exercise, 20 control) Resistance and aerobic exercise (6 months) Control (manual therapy and motor control exercise) No change in whole IVD T2 times, ADC and height between groups after controlling for false positives Owen et al. 2021 Observational, cross-sectional Participants from a wide range of sports and non athletic controls (n=379,308 athletes, 71 control) No intervention -Sport (baseball, swimming, basketball, kendo, soccer, running) -No sport (control) -Average lumbar IVD height relative to vertebral body height 7.6% greater than controls in basketaball (p=.001) and swimming (p=.001) -Individual IVD height relative to vertebral body height greater than controls in basketball (6.3-14%, p≤.029) and swimming (7.6-15%, p≤010) -Individual IVD height relative to vertebral body height greater than controls at L2 to L3 in soccer (8.7%,p=0.36) and at L3 to L4 for baseball (7.6%, p=.011) Cartilage Armagan et al. 2015 Prospective, randomized single blind Ambulatory patients aged 45-70 years with idiopathic knee OA (n= 70, 30 home exercise, 40 glucosamine sulphate) Home exercise program (6 months) Glucosamine sulphate group Improvement cartilage thickness (pretreatment 2.5 (0 min–4 max) post-treatment 2 (0 min –3 max), p<0.05) in medial femoral condyle in the home exercise group Cotofana et al. 2010 RCT Women aged 45-55 years of age (n=38, 18 endurance, 15 strength, 5 autogenic) Exercise intervention (3 months) -Strength training -Endurance training -Autogenic training (control) No significant change in knee (patellar, medial tibia and medial femur) cartilage morphology Hinterwimmer et al. 2014 Interventional, non randomized Asymptomatic marathon beginners (n= 10, 5 males and 5 females) Supervised running program and a marathon ( 6 month) No comparison - Lateral femur cartilage thickness decrease 1.7 ± 1.6 % (p = 0.010). - Lateral femur cartilage volume decrease 3.2 ± 3.0 % (p = 0.012) Koli et al. 2015 RCT Postmenopausal women with mild tibiofemoral OA (n=80, 40 exercise, 40 control) Aerobic/step aerobic exercise (12 months) No training (control) Improvement in total patellar cartilage (baseline 47.9 (SD 7.9), post-treatment -3.8 (95% CI: -6.4 to -1.9), p=.018) Küçük et al. 2018 Experimental, prospective comparative Women with primary bilateral knee OA (n=45, 15 aerobic, 15 isokinetic, 15 isometric) Exercise intervention (4 weeks) -Isokinetic exercise -Aerobic exercise -Isometric exercise Change in patellar cartilage volume (pre-treatment 2.24±0.29 mm³ to 2.35±0.34 mm³ post-treatment, p=0.036) in isometric group Munukka et al. 2016 Two experimental arm RCT Women aged 60-68 years with mild knee OA (n=87, 43 aquatic group, 44 control group) Aquatic resistance training (4 months) Usual care (control) Decrase in T2 times (-1.2 ms, 95% CI:-2.2 to-0.2,p=0.021) in the aquatic group compared to controls in the medial femoral cartilage Bone Alghadir et al. 2016 RCT Healthy subjects aged 30-60 (n=100, 47 men 53 women) Aerobic exercise (12 weeks) No comparison -Increase in BMD at the hip (normal 0.97±0.18, p<0.05, osteopenic 0.89±0.1, p<0.01, osteoporotic 0.98±0.27, p<0.01) -Increase in BMD at the spine (normal 0.96±0.12, p<0.05, osteopenic 1.6±0.35, p<0.01, osteoporotic 1.93±0.45, p<0.01) Bailey & Brooke-Wavell 2010 RCT Premenopausal woman (n= 85, 21 EX2, 22 EX4, 22 EX7, 20 CON) Exercise intervention (6 months) -Plyometric Exercise two days a week (EX2) -Plyometric exercise four days a week (EX4) -Plyometric exercise seven days a week (EX7) -Control (CON) -Increase in femoral neck BMD group EX7(+1.7 (+0.7-2.7, ) than in CON (-0.3 (-1.2-0.5), p= 0.003) and EX2(+0.2 (-0.8-1.2), p= 0.015) Bailey et al. 2010 Cross sectional, descriptive Healthy caucasian males (n=281) No intervention -Lifetime loading history (Low, mid, high tertile) -Higher BMC at mid-femur in the mid (5.639± 0.590,p<0.05) and high (5.771± 0.658, p<0.01) tertile compared to low -Higher BMC at mid-tibia in the mid (4.266± 0.534, p<0.01) and high (p<0.001) tertile compared to low Bolton et al. 2012 Single blind RCT Post menopausal women with osteopenia (n=39, 19 EX, 20 CON) Resistance, impact and balance, EX (52 weeks) -Control (CON) -Non-significant 0.5% increase in the EX group and a non-significant 0.9% loss in the CON group in total hip BMD -Mean difference in change between groups of −0.012 g/cm2 (95% CI −0.022 to −0.002 g/cm2, p= 0.02) at total hip BMD Detter et al. 2013 Prospective controlled study Children aged 6-9 (n=2395, 808 INT, 1587 CON) Standard physical activity classes (5 years) -Daily physical education (INT,200 min/week) -Daily physical education (CON,60 min/week) -Higher annual gain in spine BMD (0.045 (0.038, 0.050) p<0.001), FN BMC (0.36 (0.32, 0.40) p=0.02),and FN area(0.21 (0.18, 0.24) p=0.03) in INT girls compared to CON - Larger tibial cortical BMC (2.8 (2.7, 2.9) p=0.03), larger tibial cortical area (236 (225, 245) p=0.02), and larger radial CSA(130 (122, 139) p=0.04) in INT girls compared to CON -Higher annual gain in spine BMD (0.028 (0.025, 0.030p=0.01) in INT boys compared to CON Dowthwaite et al. 2007 Cross sectional, cohort Premenarcheal gymnasts (n=56) No intervention -Gymnasts -Non-gymnasts -Higher BMC at the ultradistal radius (0.90 (0.82–0.98), p≤0.001), areal BMD (0.365 (0.343–0.386), p≤0.001), mean periosteal width 16.20 (15.43–16.96), p<0.05) at the ultradistal radius in the gymnast group -Higher BMC (1.09 (1.03–1.16), p≤0.001),areal BMD (0.511 (0.487–0.535), p<0.05), mean periosteal width (10.62 (10.23–11.01), p≤0.001), and cortical cross sectional area (53.76 (50.66–56.86), p≤0.001) at 1/3 distal radius in the gymnast group Draghici et al. 2019 Cross sectional, cohort Men with SCI (n=13) FES rowing No comparison -Interaction between total distance rowed,TSI and peak foot force in trabecular thickness (R2=0.72,p<0.01) Du et al. 2021 RCT Post-menopausal women (n=10) High impact unilateral exercise (6 months) No comparison -Increase in trabecular number (basline1.70 ± 0.13, post intervention1.78 ±0.20; time × leg interaction p= 0.043) Ducher et al. 2004 Cross sectional Regional level tennis players (n=57, 33 men, 24 women) No intervention -Distal ulna and radius from playing arm -Distal ulna and radius from non playing arm -Higher BMC total in radius ( 14.98 ± 7.03, p< 0.0001) and in ulna (13.44 ± 7.36,p < 0.0001) in the playing arm Ducher et al. 2011 Cohort, prospective Competitive female tennis players aged 10 to 17 (n=45,13 premenarcheal (pre/peri), 32 postmenarcheal (post)) Tennis playing (12 months) -Distal ulna and radius from playing arm -Distal ulna and radius from non playing arm -Increase in the pre/peri group in BMC (20.6±10.0,p<.001), ToA (11.7±6.6, p<.001) and CoA (19.9±11.7, p<.001)in the playing arm compared to the non playing arm -Increase in the post group in BMC (19.2±10.2,p<.001) and ToA (10.1±5.0, p<.001) in the playing arm compared to the non playing arm Ducher et al. 2009 Cross sectional, descriptive Pre, peri and postpubertal competitive male tennis players (n=43) No intervention -Tennis(sport) -Higher cortical area (35.6 ± 10.3,p<0.01) in prepubescent vs peripubescent boys -Higher cortical area (66.1 ± 18.6,p< 0.01) in postpubescent vs peripubescent boys Greene et al. 2009 RCT Prepubertal girls aged 6-10 (n=42, 13 CON, 13 LD, 13 HD) Single leg drop landing exercise (8 months) -Low drop 14 cm (LD) -High drop 23 cm (DP) -Control (CON) - No change in osteogenic adaptations in bone geometry, biomechanical properties or bone strength index Harding et al. 2020 Semi-randomized RCT Middle-aged and older men ≥ 45 years of age with osteopenia or osteoporosis (n=93, 34 HiRIT, 33 IAC, 26 CON) High intensitiy progressive resistance and impact training, HiRIT (8 months) -Isometric axial compression (IAC) -Control (CON) -Increase in HiRIT in FN medial cortical thickness compared with CON (5.6 ± 1.7% versus -0.1 ± 1.9%, p= 0.028) and IAC (5.6 293 ± 1.7% versus 0.7 ± 1.7%, p= 0.044) -Improvement in FN cortical volume (0.09 ± 0.05 cm3, p = 0.041) in HiRIT -improvement in HiRIT compared to CON for distal tibia total BMC (0.1 ± 0.3% 286 versus -3.0 ± 0.4%, p< 0.001), total vBMD (0.0 ± 0.3% versus -0.8 ± 0.3%, p= 0.050), total area (0.0 ± 0.4% versus -2.1 ± 0.5%, p= 0.001), total BSI (0.1 ± 0.4% versus -3.9 ± 0.5%, p< 0.001), trabecular BMC (0.4 ± 0.4% versus -1.8 ± 0.5%, p= 0.001), trabecular area (0.2 ± 0.5% versus -1.6 289 ± 0.5%, p= 0.013), and trabecular BSI (0.7 ± 0.5% versus -1.9 ± 0.6%, p= 0.001) Hasselstrøm et al. 2008 Non-randomized RCT Pre-school children (n=379, 135 boys and 108 girls INT, 62 boys and 76 girls CON) Standard physical activity classes (3 years) -Standard physical activity classes with higher volume (180min/week, INT) -Standard physical activity classes with normal volume (90 min/week,CON) -Increase in distal forearm BMC(2.14±0.34,p=0.04) in INT girls compared to CON girls -Increase in distal forearm scanned area (7.42±0.82, p= 0.005) in the INT girls compared to CON girls Heinonen et al. 2002 Observational, cross sectional Female national and international level weightlifters and powerlifters(n=14, 14 CON) No intervention -National and international level weightlifters and powerlifters - Aged matched, relatively active (CON) -Difference in distal femur total density ( 301.2 (30.0), p=0.040) in favour of the weightlifing group -Difference in cross sectional area at the distal radius (101.9(28.0),p= 0.029) and in the radial shaft (88.8 (16.5), p=0.001) in favour of the weightlifing group -Difference in cortical wall shaft in the radial shaft (3.6 (0.46), p=0.037) in favour of the weightlifing group -Difference in cortical area at the tibial midshaft (305.7(35.0), p=0.034) in favour of the weightlifing group Hughes et al. 2018 Observational, longitudinal U.S. army female recruits (n=91) Basic combat training (8 weeks) No comparison -Increase in mean trabecular thickness (1.13% (0.76, 1.50); p < 0.001), trabecular number (1.21% (0.48, 1.94); p < 0.05), trabecular bone volume/total volume (1.87% (1.31, 2.43); p < 0.001), trabecular BMD (2.01% (1.44, 2.58); p < 0.001), and cortical thickness (0.98% (0.38, 1.58); p < 0.001) at the tibia Karinkanta et al. 2007 RCT Women aged 70-79 (n= 149, 37 RES, 37 BAL, 39 COMB, 37 CON) Exercise intervention (12 months) -Resistance training group (RES) -Balance-jumping training group (BAL) -Resistance training and balance-jumping training (COMB) -Non-training control group (CON) -No effect in BMD at femoral neck or tibial shaft Kukuljan et al. 2011 RCT Healthy males aged 50-79 (n= 180,45 EX+FM, 46 EX, 45 FM, 44 CON) Exercise intervention and supplementation (18 months) -Exercise plus fortified milk (EX+FM) -Exercise (EX) -Fortified milk (FM) -Control (CON) -Increase in femoral neck BMD (1.9% (95% CI, 1.2, 2.5)), CSA (1.8% (95% CI, 0.8, 2.7)) in the exercise group -Increase in lumbar spine trabecular BMD (2.2% (95% CI, 0.2, 4.1)) in the exercise group Lambert et al. 2020 Single blind RCT Inactive healthy young adult women aged 18-30 (n=22, 10 impact training, 12 resistance training) Two exercises regimes (10 months) -High intensity progressive impact training (IT) -High intensity progressive resistance training (RT) -Improvement in BMD in the dominant radius for both IT (0.033±0.015 g/cm2, p= 0.046) and RT (0.037±0.014 g/cm2, p= 0.015) -Improvement in trabecular (1.86 ± 0.90 % versus -1.30 ± 0.81%, p= 0.029), distal radius (8.55 ± 2.26% versus 1.50 ± 2.04%, p= 0.040) and total BSI bone strenght index (15.35 ± 2.83% versus 2.67 ± 2.55%, p = 0.005) density in the non dominant limb in the IT group compared to RT -Improvment in cortical content (2.63 ± 1.08 mg, p= 0.025), density (29.53 ± 7.70 mg/cm3, p= 0.001) and cortical thickness (0.06 ± 0.02 mm, p= 0.019) in the non dominant limb in the RT group compared to IT -Improvement for dominant FN trabecular BMC (9.64 ± 5.29% versus -10.74 ± 5.86%, p= 0.024), total BMC (8.06 ± 5.22% versus -11.15 ± 5.77%, p = 0.030),and cortical BMD (3.68 ± 1.99% versus -4.14 ± 2.20%, p = 0.021) in the RT group compared to the IT -Improvement in dominant tibial shaft cortical area (3.41± 1.31 mm2, p= 0.017), periosteal circumference (0.38 ± 0.15 mm, p= 0.018) in the RT group -Improvement in cortical thickness for both RT (0.05 ± 0.02 mm, p = 0.021) and IT (0.05 ± 0.02 mm, p = 0.047) Lang et al. 2014 Prospective, non randomized Healthy men and women (n=22, 8 ABBAD, 7 SQDL, 7 COMBO) Exercise intervention (16 weeks) -Standing hip abduction/adduction (ABADD) -Squat/deadlift (SQDL) -Combination (COMBO) -Increase in the volume of the trochanteric cortical region (4.1%,p<0.01) in the ABADD group -Increase in integral vBMD, cortical vBMD, and cortical volume (1.6% to 3.4%,p<0.05) in the SQDL group in the femoral neck -Increase in vertebral integral BMD (3.1%,p<0.05) and spinal trabecular BMD (7.0%,p<0.05) in the SQDL group Marques et al. 2013 Prospective, non randomized Caucasian older adults (n=40, women 20, 20 men) Exercise intervention (resistance and odd impact, 32 weeks) No comparison -Increase trochanteric (0.648±0.080 women, 0.774 0.114 men,p<0.001) intertrochanteric (1.041±0.139 women, 1.172±0.163 men, p=0.005) total hip (0.872±0.111 women, 1.006±0.138, p=0.001) lumbar spine (0.896±0.129 women, 1.065±0.172 men, p= <0.001) and FN (0.705±0.104 women, 0.821±0.115 men, p=0.002) BMD Marques et al. 2011 RCT Caucasian older women (n=60, 30 ET, 30 CON) Multicomponent exercise training (8 months) -Exercise training (ET) -Control (CON) -Increase in BMD FN (0.717±0.085,p=0.008) in the ET group Milliken et al. 2003 Prospective, comparative Postmenopausal women with and without HRT (n=94, 26 EX, 30 NO, 17 EX+HRT, 21 HRT) Exercise intervention and hormone therapy (12 months) -Exercise (EX) -No exercise, no HRT (NO) -Exercise+ HRT (EX+HRT) -No exercise, HRT (HRT) -Increase in BMD at the greater trochanter (3.0 ± 7.7,p= 0.03) in the EX+HRT at 12 months -Increase in BMD at Ward's triangle ()1.0 ± 8.7, p= 0.04) in EX at 12 months Morse et al. 2019 Comparative RCT Non ambulatory men and women with SCI (n=69, 35 EX, 34 EX+ZA Exercise intervention and supplementation (12 months) -Functional electrical stimulation rowing exercise (EX) -Functiona electrical stimulation rowing exercise + Zoledronic acid (EX+ZA) -Greater CBV in proximal tibia metaphysis (345±109 mm3,p=0.006) and in distal femoral metaphysis (471±225 mm3, p=0.05) in EX+ZA compared to EX Nilsson et al. 2013 Observational, cross sectional Active men (n= 361, 106 RE, 78 SC, 177 CON) No intervention -Resistance exercise (RE) -Soccer (SC) -Non exercise (CON) -Higher BMD at FN (1.26±0.17p<0.001) lumbar spine (1.36±0.15, p<0.001) larger cross sectional cortical size at the tibia (310±34, p<0.001) trabecular number (2.25±0.27, p<0.001) trabecular thickness (90.8±11.0p<0.001) in SC group O'Leary et al. 2019 Observational, longitudinal British male infantry recruits (n=42) British Army's infantry basic military training course (13 weeks) No comparison -Increase in areal BMD for total body (P = 0.031, dz= 0.36) and arms (P = 0.001, dz= 0.57) -Increase in total BMD(dominant leg 351± 41, p< 0.05, non-dominant leg 233± 25, p< 0.05) trabecular BMD(dominant leg 232± 25, p< 0.05, non-dominant leg 233± 25, p< 0.05) and cortical BMD(dominant leg 888± 26, p< 0.05, non-dominant leg 896± 26,p< 0.05) Pang et al. 2006 RCT Community dwelling individuals with stroke (n=63, 32 INT, 31 CON) Intensive exercise program (19 weeks) -Aerobic, balance and resistance exercise (INT) -Upper extremity exercise(CON) -Increase in trabecular BMC (231.9±56.,p=0.048) in the INT group compared to the CON Rantalainen et al. 2011 Observational, cross sectional Premenopausal women athletes (n=180, 60 HI, 47 OI, 15 HM, 16 RLI, 42 RNI) and 41 non ethletic referent No intervention -High impact sport (HM) -Odd impact sport (OI) -High magnitude sport (HM) -Repetitive, low impact sport (RLI) -Repetitie, non impact (RNI) -Non athletic -No significant region-by-exercise group interactions (F= 1.587, P= 0.140) in the mean radial cortical BMD -OI groups had 1.5 to 2.6% (p<0.05) lower cortical BMD than referents at all four sectors (posterior, lateral, anterior and media) at the tibial shaft -RLI group had 1.0% lower BMD at the anterior sector (p<0.05) than referents at all four sectors (posterior, lateral, anterior and media) at the tibial shaft -HM group had 1.2% lower BMD at the lateral sector (p<0.05) than referents at all four sectors (posterior, lateral, anterior and media) at the tibial shaft Specker & Binckley 2003 Randomized, placebo controlled, partially blinded Children aged 3-5 (n=239,57 FMC, 57 FMP, 62 GMC, 62 GMP) Exercise plus supplementation or placebo (1 year) -Fine Motor+ Calcium (FMC) -Fine Motor+Placebo (FMP) -Gross Motor+Calcium (GMC) -Gross Motor+Placebo (GMP) -Increase in leg BMC in GMC (40.9±1.3, p=0.05) group compared to other groups -Increase in periosteal (49.5±0.7 GMC,49.9±0.7 GMP, p= 0.03) and endosteal (40.7±0.9 GMC, 41.7±0.9 GMP, p=0.05) circumference in both GMC and GMP compared to other groups Vainionpää et al. 2007 RCT Women aged 35 to 40 (n= 5161, 60 EX, 60 CON) Impact and plyometric exercises EX (12 months) -Control (CON) -Increase in midfemur bone circumference 0.2% (95% CI: 0.01% to 0.35%; p= 0.033) in the EX group compared to CON -Subjects in the highest quartile (> 66 exercise sessions during the 12 months) showed a 1.2% (95% CI: 0.2 to 2.2; p= 0.03) gain in bone circumference and a 0.5% (95% CI: 0.0 to 0.9; p= 0.04) gain in cortical CSA compared to the subjects in the lowest quartile (< 19 sessions) at proximal tibia Valdimarsson et al. 2006 Prospective non randomized School aged girls (n=103, 53 INT, 50 CON) Ordinary physical activity in the Swedish school curriculum (1 year) -Physical activity in the Swedish school curriculum with higher volume (40min/day, INT) -Physical activity in the Swedish school curriculum with higher volume (60min/week, CON) -Higher BMC in lumbar spine (2.4 ± 1.1,p=0.01) and L3 (.94 ± 0.63, p<0.001) in the INT group compared to the CON group -Higher BMD in the lumbar spine ( 0.044 ± 0.0, p<0.001) and L3 (0.047 ± 0.0,p<0.001)in the INT group compared to the CON group -Higher bone width in L3 (0.17 ± 0.12,,p<0.001)in the INT group compared to the CON group Watson et al. 2015 Single blind RCT Postmenopausal women aged 60+ with low bone mass (n=28, 12 HiPRT, 16 CON) High-intensity progressive resistance training HiPRT (8 months) -Control (CON) -Improvment FN BMD (0.3±0.5 % vs−2.5±0.8 %,p=0.016) and LS BMD(1.6±0.9 % vs−1.7±0.6 %,p=0.005) in the HiPRT group Winters-Stone et al. 2014 Single blind RCT Men with prostate cancer (n= 51, 29 POWIR, 22 FLEX) Progressive, moderate-intensity resistance + impact training, POWIR (12 months) -Flexibility training (FLEX, used as control) - Preservertion of L4 BMD in the POWIR group (−0.4%, p=0.03) compared to loss (−3.1%) in controls (FLEX) Winters-Stone et al. 2013 RCT Women breast cancer survivors with treatment related menopause (n=71, 35 POWIR, 35 FLEX) Progressive, moderate-intensity resistance + impact training POWIR (12 months) -Flexibility training (FLEX, used as control) -Change in both spine (ITTresults—coefficient on slope of time00.009, SE00.004,t(48)02.21, p=0.032) and femoral neck BMD (ITTresults—coefficient on slope of time00.011, SE00.004,t(48)03.19, p=0.004)among women with 1+ years past onset of menopause in the POWIR group Winters-Stone, Snow 2006 Prospective, non randomized Premenopausal woman (n= 35, 19 LOWER, 16 UPPER+LOWER, 24 CON) Exercise intervention (12 months) -Lower body resistance plus jumping exercise (LOWER) -Upper and lower body resistance plus jumping exercise (UPPER+LOWER) -Control (CON) -Increase in greater trochanter in UPPER+LOWER (2.2± 2.8, p< 0.05) and LOWER (2.6± 2.5,p< 0.05) compared to CON -Increae in spine BMD in UPPER+LOWER (1.3± 3.7,p< 0.05.) compared to CON and LOWER Wochna et al. 2019 Non-randomized RCT Post-menopausal women aged 54-65 (n=18, 9 T, 9 CON) Aqua fitness training (6 months) -Aqua fitness training (T) -No physical activity (CON) -No effect in in BMD at left hip, lumbar spine or whole body Ligament Grzelak et al. 2012 Observational, descriptive Male weightlifters and age matched controls (n=28, 9 W, 19 CON) No intervention -Weightlifting (W) -Age matched control (CON) -Higher mean cross sections in W (71.7, 52.9–111.2) than CON (40.56, 23.83–59.1) for ACL -Higher mean cross sections in W (64.48, 52–88.1) than CON(44.98,31.3–71) for PCL Myrick et al. 2019 Observational, prospective Division-I female collegiate soccer players (n=17) 2013-2014 soccer season No comparison -Mean ACL volume increased from pre to pos-season (pre1426 cc (SD= 288), post 556 cc (SD = 269), p=.006) - Greater volume increase in the right than the left knee (right 211 cc, left 48 cc, p =.047) Tendon Arampatzis et al. 2007 Interventional non-randomized Not strength trained adults (n=21, 11 INT, 10 CON) Isometric plantarflexion (14 weeks) -Exercise intervention (One leg low strain magnitude and one leg high strain magnitude, INT) -No exercise control (CON) -Increase in AT CSA at 60% and 70% of tendon length in the high strain leg (p<0.05) -Higher AT CSA at 60% and 70% of tendon length in the high strain leg compared to the low strain leg (p<0.05) Bohm et al. 2014 RCT Male adults (n=39, 14 G1, 14 G2, 13 CON) Plantar flexion exercises (14 weeks) -Plantar flexion contraction+reference protocol leg+ contralateral one legged jumps (G1) -Plantar flexion contraction+reference protocol leg+ contralateral12s isometric plantar flexion (G2) -No especific training (CON) -Increase in AT stiffness (p<0.001) in G1 -Increase in AT CSA in G1 reference leg protocol in the 30% to 100% part of the tendon -Increase in the AT CSA in G1 one legged jumps leg in the 20% to 30% part of the tendon -Increase in AT stiffness (p<0.001) in G2 -Increase in AT CSA in G2 both in reference leg protocol and isometric plantar flexion leg in the 30% to 100% part of the tendon Epro et al. 2019 Cross sectional descriptive Healthy young participants (n= 67, 35 males, 32 females) No intervention -High jump (HJ) -Triple jump (TJ) -Long jump (LJ) -Pole vault (PV) -Greater AT stiffnes in the take off leg compared to the swing leg (p< 0.001) -Higher AT stiffness in males compared to females (p< 0.001) -Lower AT stiffness values in PV compared to HJ (p= 0.038), TJ (p= 0.041)and LJ (p= 0.035) Epro et al. 2017 Interventional non-randomized Old female adults (n=34, 21 INT, 13 CON) Isometric plantar flexion (14 weeks and 1.5 years follow up) -No exercise control (CON) -Higher AT stiffness in INT ( 598.2±141,p=0.01) at 14 weeks -Higher AT CSA in INT (72.0±11.5,p=0.007)at 14 weeks -Higher AT stiffness in INT (637.1±183,p<0.001) at 1.5 years -Higher AT CSA in INT (71.5±11,p<0.01) at 1.5 years Karamanidis & Epro 2020 Observational cross sectional and longitudinal (1 year and 4 years) Healthy young participants (n=91, 67 EL ,24 CON) No intervention -Elite international level jumping track and field athletes (EL) -Young healthy recreationally active (CON) -Higher AT stiffness in the EL group compared to CON (653 ± 187 vs 570 ± 131, p= 0.048) irrespective of analyzed leg -Higher AT stiffness in the take off leg compared to the swing leg in the EL group (675 ± 195 vs. 630 ± 186, p <0.001) -Not statistical significant tendencies for greater fluctuation in tendon stiffness in the EL group compared to the CON group at 1 year (p= 0.074) Milgrom et al. 2014 Observational longitudinal Basic training infantry recruits (n=55) Basic infantry training (6 months) No comparison -Increase in AT CSA in the right leg (50.2 ± 9.6,p=.037) and left leg (51.1 ± 8.3, p=.013) Werkhausen et al. 2018 Interventional non-randomized Recreationally active volunteers (n=21, 11 INT, 10 CON) Explosive isometric unilateral plantar flexion, (10 weeks) - No exercise (CON) -Increase in AT stiffness (459 ± 147, p< 0.05) in the INT group Westh et al. 2007 Observational descriptive Healthy subjects (n=30, 10 MR, 10 FR, 10 NR) No intervention -Male runners (MR) -Female runners (FR) -Female non-runners (NR) -Greater PT CSA in the proximal and distal portion in MR compared to FR and NR (p<0.01) -Greater PT CSA in the mid portion in MR compared to FR (P<0.05) and NR (p<0.01) -Greater distal PT CSA compared to proximal part in MR (p<0.01) and NR (p<0.05) -Smaller AT CSA in the distal part in FR and NR compared to MR (p<0.01) Zhang et al. 2015 Cross sectional descriptive Healthy male subjects (n=40, 10 SED, 15 VOL, 15 BAS) No intervention -Sedentary subjects (SED) -Volleyball players (VOL) -Basketball players (BAS) -Higher PT CSA in the VOL (dominant leg 127.9 ± 16.2, non-dominant leg 129.4 ± 17.4) and BAS (dominant leg 129.5 ± 32.6, non-dominant leg 133.7 ± 28.3) group compared to SED (p < 0.05) Muscle Bickel et al. 2011 Interventional non randomized Healthy adults (n=70, 31 OLD, 30 YOUNG) Knee extensor resistance training (16 weeks) -60-75 year old participants (OLD) -20-35 year old participants (YOUNG) -Increase in TLM in both OLD and YOUNG (11.164 ±573 and 13,128 ±508,p< 0,05) -Increase in Type II fibers CSA in both OLD and YOUNG (4636 ± 298 and 5917 ±264, p< 0,05) -Increase in Type I fibers CSA in both OLD and Young (5237 ± 241 and 4991 + 178, p< 0,05) Fernandez-Gonzalo et al. 2014 Interventional non randomized Healthy subjects (n=32, 16 men, 16 women) Bilateral flywheel supine squat resistance exercise (6 weeks) -Men -Women -Increase total thigh muscle mass in both men (1,031.1 ± 64.4, p<0.05) and women (628.5 ± 50.2, p<0.05) Franchi et al. 2015 RCT Recreationally active healthy young men (n=10) Leg press resistance exercise (4 weeks) -Eccentric leg contraction only (ECC) -Concentric leg contraction only (CON) -Increased FL in ECC (5±0.6%,p<0.001) than CON -Increased PA in CON (7±0.9%,p<0.001) than ECC -Increase in muscle thickness in both ECC and CON (7.5±1.6% and 8.4±1.4%,p<0.001) -Increase in TLM in both ECC and CON (2.3±0.5% and 3±0.6%,p<0.01 and p<0.001) Franchi et al. 2014 Interventional non randomized Young men ( n=12, 6 ECC, 6 CON) Leg press resistance exercises (10 weeks) -Eccentric exercise (ECC) -Concentric exercise (CON) -Increase in VL muscle volume in both ECC (6± 0.4%,p<0.0001) and CON (8±0.5%, p<0.0001) -Increase in FL in ECC(12±2%,p<0.0001) compared to CON -Increase in PA in CON (30±0.5%,p<0.0001) compared to ECC -Difference in VL regional hyperthrophy in mid portion between ECC and CON (7±1% and 11±1%, p<0.01) -Difference in VL regional hyperthrophy in distal portion between ECC and CON (+8±2% and +2±1.5%, p<0.05) -Similarities in VL regional hyperthrophy in the proximal portion between ECC and CON (-1±1% and -0.5±1%) Häkkinen et al. 2002 RCT Premenopausal women with FM (n=21, 11 FMT, 10 FMC and 12 CON) Total body resistance training (21 weeks) -Healthy women (CON) -Increase in CSA in quadriceps femoris in FMT at 5–12/15 femur length (p< 0.05–0.01) -Increase in CSA in quadriceps femoris in CON at 3–12/15 femur length (p< 0.05–0.001) Holm et al. 2008 Interventional non randomized Healthy young sedentary men (n=11) Unilateral resistance training (12 weeks) -Light load (LL) -Heavy load (HL) -Higher increase in total CSA at quadriceps in HL (7.6±1.4%p<0.05)compared to LL Marzilger et al. 2020 RCT Young active men (n=47, 33 EX, 14 CON) Eccentric knee extensor contractions (33 training sessions) -No exercise (CON) -p45: angular velocity 45°/s -p120: angular velocity 120°/s -p210: angular velocity 210°/s -p300: angular velocity 300°/s -Increase in FL in all exercise protocols (p45: 5.7%, p= 0.006, p120: 4.2%, p = 0.004, p210: 3.6%, p = 0.024, p300: 7.6%, p = 0.002) compared to CON -Increase in VL volume irrespective of protocol (p< 0.001)