Open-access Molecular identification of Diptera of forensic importance with the gene (COI Barcode), La Paz Bolivia

Abstract

One of the difficulties encountered is the correct identification of insects associated with cadaveric decomposition, which has led to the search and generation of new tools in molecular biology that facilitate the determination of specificities for the modification of the Post-Mortem Interval in an effective and accurate from immature stages; the collection and morphological taxonomy of Diptera were made in the first instance and then the Bar Code System (COI Barcode) was used for the molecular identification of problem insects by means of the mitochondrial COI gene in any phase of the biological cycle. Identifying three species of adults with a probability of 100% correspondence; the specimens: Sarconesia versicolor of the Calliphoridae Family and Fannia sp., were not found in the world databases of the GenBank and the Boldsystems, being necessary to update them by chronological succession patterns of cadaveric fauna in different geographical areas, whose practice would be applied in criminal investigations.

Key words: Bar code; molecular taxonomy; IOC and dipterans

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Asociación Costarricense de Medicina Forense Apdo. 187-3007 San Joaquín de Flores , Heredia Costa Rica, San Joaquin de Flores, Heredia, CR, 187-3007, 2277-4128 - E-mail: cabarca@racsa.co.cr
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