<span name="style_bold"><span name="style_italic">In vitro </span>culture of <span name="style_italic">Geophila macropoda </span>(Ruiz &amp; Pav. DC) from zygotic embryos. </span>The goal of this research was to develop a protocol for the <span name="style_italic">In vitro </span>culture of <span name="style_italic">Geophila macropoda </span>(Ruiz &amp; Pav. DC) from zygotic embryos. <span name="style_italic">in vitro </span>culture of <span name="style_italic">Geophila macropoda</span>. The work was conducted in Guácimo, Limón, Costa Rica between June 2006 and February 2007. For the <span name="style_italic">in vitro </span>establishment phase, the explants used were microcuttings and zygotic embryos. Bacterial contamination prevented the establishment of high percentages of plant material, whereas the use of embryos resulted in a response as high as 89%. For shoot proliferation, five concentrations of benzilaminopurine (BAP) were tested. The best results were obtained with 2 mg/l BAP with 13.4 new seedlings from an original vitroplant. Rooting was accomplished in a half Murashige &amp; Skoog medium, without adding any plant growth regulator. During the acclimatization period the survival rate was 100%.establishment phase, the explants used were microcuttings and zygotic embryos. Bacterial contamination prevented the establishment of high percentages of plant material, whereas the use of embryos resulted in a response as high as 89%. For shoot proliferation, five concentrations of benzilaminopurine (BAP) were tested. The best results were obtained with 2 mg/l BAP with 13.4 new seedlings from an original vitroplant. Rooting was accomplished in a half Murashige & Skoog medium, without adding any plant growth regulator. During the acclimatization period the survival rate was 100%.

Mouse ear; micropropagation; Benzylaminopurine; cover crop